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sgrna expression vector pcrispria v2  (Addgene inc)


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    Structured Review

    Addgene inc sgrna expression vector pcrispria v2
    Sgrna Expression Vector Pcrispria V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+vector+pcrispria+v2/pCRISPRia-v2+(Plasmid+%2384832)/pm41422267-207-34-38
    Average 96 stars, based on 143 article reviews
    sgrna expression vector pcrispria v2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI - SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832 ; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( Horlbeck et al., 2016 )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708 ; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( Datlinger et al., 2017 )).

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI – SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( )).

    Generated:

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI - SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832 ; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( Horlbeck et al., 2016 )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708 ; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( Datlinger et al., 2017 )).

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI – SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( )).

    Blocking Assay:

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI - SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832 ; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( Horlbeck et al., 2016 )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708 ; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( Datlinger et al., 2017 )).

    Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons
    Article Snippet: .. The secondary screening vector pMK1334 was generated as follows: The PpuMI – SnaBI fragment of CROPseq-Guide-Puro was replaced with a gene block encoding the mU6-BstXI-BlpI-optimized sgRNA backbone fragment from our sgRNA vector pCRISPRia-v2 (Addgene plasmid # 84832; http://n2t.net/addgene:84832 ; RRID:Addgene_84832 ( )) to obtain pMK1332. .. CROPseq-Guide-Puro was a gift from Christoph Bock (Addgene plasmid # 86708; http://n2t.net/addgene:86708 ; RRID:Addgene_86708 ( )).



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